The Potential Role of Microbes in The Three Sisters: Part I, The Good Microbes by Daniel Hayden

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The potential role of microbes in The Three Sisters, Part I: The good microbes

By Daniel Hayden

Despite not being visible to the human eye, microorganisms like fungi, bacteria, and viruses play such pivotal roles in our world. All of human history has been dictated by microbes, from fermentation of our foods to pandemics like COVID-19. Plants are no different, as interactions with microbes have driven plant evolution leading to many of the interesting aspects of plants we see today. Recently, there is a growing interest in how microbes influence plants in agriculture and how scientists can make the microbes work for us to produce better crops and healthier soil.

The development large-scale agriculture mainly focused on the crop, breeding and engineering to increase yield and create uniformity. It makes sense… the crop is producing the fruit/seeds so you need to protect the crop at all costs through fertilizers, pesticides, and reducing weed competition via tillage. However, this thinking often overshadowed other aspects like soil health and microbes, which are inherently connected.

In this post, I’ll discuss why traditional forms of agriculture don’t need chemical inputs, machinery, or genetically-engineered crops, but rely on the time-tested interactions between plants and microbes. The example I will use has existed in North America for time immemorial and practiced by Indigenous peoples: The Three Sisters.

The Three Sisters mound system, is a traditional polyculture of corn, beans, and squash grown on a mound of soil. Each crop has it’s own job within the polyculture: corn provides structure for beans to climb up to reach sunlight, beans provide nitrogen via an association with nitrogen fixing bacteria, and squash provide shading to retain soil moisture and suppress weeds. This is a very general rundown of the Three Sisters, as there are many stories and knowledge throughout many cultures. This post is focused more on the microbial relationships, so I recommend reading the plethora of knowledge online or asking family and community members if you want to gain more general insight.

All crops in the Three sisters form mycorrhizal associations, as not forming mycorrhiza is more of the exception. The main nutrients taken up by mycorrhizal fungi are nitrogen and phosphorus, which are the important ones for plant health and development. In the Three Sisters, a common practice is actually placing fish fertilizer in the mound before planting. The mycorrhizal fungi would optimally utilize these nutrients for these crops, as roots cannot reach all nutrients in the soil.

This is a diagram of a root, where the red represents areas where nutrients have been depleted and the green is sources of nutrients. On the right side, the brown mycorrhizal fungi extends into these areas.

Furthermore, mycorrhizal fungi produce proteins called glomalin, which influence soil structure and nutrient availability, but how long it takes to see these results is probably several years. Most changes in soil like nutrients or even structure generally occurs over the course of years. I believe in Three Sisters mounds glomalin produced by mycorrhizal fungi improve both soil quality over time.

I hypothesize not only are these fungi playing a big role in ensuring these three crops acquire nutrients, but there is an underground network of mycorrhizal fungi allowing for potential sharing of nutrients. This makes sense in a spatial sense, as all three crops and multiples of each crop, are growing closely together on a mound of soil. The general trend I see is corn grown on the very top of the mound, beans next to the corn, and squash is planted on the side of the mound or on the ground near the mound. Inside the mound, the bean and corn roots would be closely interacting, while the squash roots would be not as close to them, but maybe further beneath the mound. The corn and bean root relationship may have developed due to how much nitrogen corn needs and how beans can provide potential sources of nitrogen. Plant ecology research indicates grasses (corn) and legumes (bean) have greatly influenced each other’s evolution.

Summary:

We know all three crops form a beneficial relationship with mycorrhizal fungi, which may help in optimal nutrient acquisition and potentially sharing nutrients between crops. Furthermore, mycorrhizal fungi influence and improve soil structure over time, which could be happening in the soil mounds. I suspect the role of beans is more temporal, year-by-year basis, meaning those nitrogen benefits directly help the bean immediately and over time the buildup of nitrogen-rich bean plant debris in the soil will add more nitrogen. These microbes are allowing these crops to optimally uptake nutrients in tandem with the crops ability to co-exist together. We need to think about the microbes as a valuable community around The Three Sisters because they have been inherently connected with these Three crops for time immemorial and can greatly influence crop and soil health.

Next Post:

No plant interacts with just one microbe, what I have described is a small part of these plant-microbe interactions. So far I have talked about beneficial microbes, but there are millions upon millions of microbes living in the soil and all over the plant, many of them we don’t even really know if they’re good, bad, or neutral. In this next post I’ll discuss the communities of microbes and the bad microbes.

Find Daniel Hayden’s blog series here on the potential role of microbes in The Three Sisters traditional polyculture mound system.

Sources:

Kapayou, D. G., et al. “Reuniting the Three Sisters: collaborative science with Native growers to improve soil and community health.” Agriculture and Human Values 40.1 (2023): 65-82.

Irving, Thomas B., et al. “A critical review of 25 years of glomalin research: a better mechanical understanding and robust quantification techniques are required.” New Phytologist 232.4 (2021): 1572-1581.

Treseder, Kathleen K., and Katie M. Turner. “Glomalin in ecosystems.” Soil Science Society of America Journal 71.4 (2007): 1257-1266.

Taylor, Thomas N., et al. “Fossil arbuscular mycorrhizae from the Early Devonian.” Mycologia 87.4 (1995): 560-573.

van Der Heijden, Marcel GA, et al. “Mycorrhizal ecology and evolution: the past, the present, and the future.” New phytologist 205.4 (2015): 1406-1423.

Vandermeer, J. H. 1992. The Ecology of Intercropping. Cambridge University Press.

Andrews, Mitchell, and Morag E. Andrews. “Specificity in legume-rhizobia symbioses.” International journal of molecular sciences 18.4 (2017): 705.

Smith, Douglas R., et al. “Surface runoff and tile drainage transport of phosphorus in the midwestern United States.” Journal of environmental quality 44.2 (2015): 495-502.

Durian, Emma, and Monica Carroll. “American Agriculture and How Mexico Pays For It: How Midwestern Agricultural Runoff Has Cost Gulf Economies.” (2022).